Targeted Homologous Recombination In Mycoplasma mycoides Subsp. Capri Is Enhanced by Inclusion of Heterologous RecA
Appl Environ Microbiol. 2010 Aug 27;
A suicide plasmid pExp1-ctpA::tetM-recAec employing recA from Escherichia coli and tetM as a selection marker was used to generate ctpA knockout mutants in Mycoplasma mycoides capri through targeted gene disruption. Inclusion of E. coli recA greatly enhanced both consistency and recovery of mutants generated by homologous recombination.