VIGOR - VIRAL GENOME ORF READER
About VIGOR
The Microbial Sequencing Center at The J. Craig Venter Institute is sponsored by the National Institute of Allergy and Infectious Diseases and is tasked with conducting high throughput sequencing of various infectious disease causing viruses including influenza, coronavirus, SARS coronavirus, rotavirus and rhinovirus.
We developed an RNA virus gene prediction program called Viral Genome ORF Reader (VIGOR) to support high throughput feature prediction and annotation. VIGOR employs an extrinsic strategy and boasts sensitivity and specificity greater than 99% for the RNA viral genomes we tested. Genome-specific features identified by VIGOR include frame shifts, overlapping genes, embedded genes, and mature peptide cleavage sites. Genotyping capability for influenza and rotavirus is built into the program.
The prediction software has been incorporated into JCVI's post-sequencing production pipeline for performing high throughput annotation and closure validation. The program is made available for public use here. The software is described in Shiliang Wang, Jaideep P. Sundaram, Elodie Ghedin, David Spiro, 2009.