- About VIGOR
- Run VIGOR
- Instructions
- Coronavirus
- Influenza virus
- Rhinovirus
- Rotavirus
- Rubella virus
- SARS
- Measles & mumps virus
- Parainfluenza & Sendai virus
- Venezuelan equine encephalitis virus & alphavirus
- Norovirus
- Metapneumovirus
- Yellowfever & Japanese encephalitis virus
- Respiratory syncytial virus (RSV)
VIGOR - VIRAL GENOME ORF READER
VIGOR (Viral Genome ORF Reader) is a web application tool to predict protein sequences encoded in small RNA viral genomes, influenza virus, rotavirus, rhinovirus, coronavirus and SARS coronavirus. VIGOR determines the protein coding sequences by sequence similarity searching against curated viral protein databases. Three output files will be generated by VIGOR prediction, gene prediction file (including predicted protein sequences and annotations), predicted FASTA format cDNA file, and the alignment between the predicted protein and the best homolog in the custom database.
To run the prediction on your sequence(s), either paste the nucleotide FASTA record(s) into the textbox or upload your FASTA file by clicking on the "Browse" button. A description of the FASTA format is provided at NCBI.
Before you submit your sequence(s), please select the virus type which you are analyzing, and enter your email address as well, therefore you can be notified once the prediction result is available.
When you click on the "Submit" button, your sequence(s) will be queued and an email will be sent to you to confirm receipt. Once the prediction has been executed, you will receive another email notification indicating how you may view the results.
Examples of VIGOR output sequence headers:
Influenza viruses.
>FJ966982.1.1 HA H1 566 Aa 1 1701 hemagglutinin [influenza A virus] >FJ966981.1.1 NA N1 469 Aa 1 1410 neuraminidase [Influenza A virus] pep_ID Gen_Symb Genotype pep_len CDS_coord product_name viral_group.
>AB049167.2 NS2 121 Aa 1 30, 503 838 nonstructural protein 2 [Influenza A virus] Pep_ID Gen_Symb pep_len CDS_coord(splicing) product_name viral_group.
SARS coronaviruses.
>445.2 7073 Aa 246..13379, 13379..21466 polyprotein orf1ab pep_ID pep_len CDS_coord(ribosomal slippage) product_name
>445.3 1255 Aa 21473 25240 spike glycoprotein precursorpep_ID pep_len CDS_coord product_name
>489.1.4 8465 9964 mat_peptide nsp4 mat_pep_ID coding_region_coord mat_pep_name