Yi-Han Lin, PhD

Staff Scientist



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Yi-Han Lin, Ph.D. joined the Infectious Disease Department of the J. Craig Venter Institute at the Rockville campus in 2018. Dr. Lin has extensive experience with bacterial pathogenesis, and her research has been focused on proteins involved at the host-pathogen interaction interface. With a variety of techniques including biochemical, biophysical, imaging, in vitro and cell-based assays, Dr. Lin has characterized different post-translational modifications of proteins that mediate microbial virulence at the host-pathogen interface.

Prior to joining JCVI, Dr. Lin completed her post-doctoral training at the National Institutes of Health, working on elucidating the molecular mechanisms underlying Legionella pueumophila pathogenesis. Dr. Lin received her Ph.D. degree in Chemistry from Emory University, and her B.S. degree in Chemistry from National Taiwan University.

Research Priorities

Identify novel antimicrobial peptides and characterize mode of action

  • Characterize the antimicrobial activity of proteolytic products of the innate immune response activated during urinary tract infection, sexually transmitted diseases, or other bacteria-mediated diseases
  • Elucidate the mode of action of novel antimicrobial peptides by MS

Host-microbial interaction interface study

  • Profile post-translational modifications (PTM) of host or microbial proteins involved in human diseases using MS to recapitulate the role of PTM at the host-pathogen interface
  • Generate deletion strains of bacterial pathogens to evaluate function of virulence factors identified by MS

Development of high-throughput MS sample analysis

  • Apply and compare different sample preparation devices to establish economical, efficient, and effective methods for quantitative proteomic and peptidomic profiling
  • Implement high-throughput sample processing methods for biomarker discovery in different disease bases

Select Publications

RavN is a member of a previously unrecognized group of Legionella pneumophila E3 ubiquitin ligases.
PLoS pathogens. 2018-02-07; 14.2: e1006897.
PMID: 29415051
Exploitation of the host cell ubiquitin machinery by microbial effector proteins.
Journal of cell science. 2017-06-15; 130.12: 1985-1996.
PMID: 28476939
Host Cell-catalyzed S-Palmitoylation Mediates Golgi Targeting of the Legionella Ubiquitin Ligase GobX.
The Journal of biological chemistry. 2015-10-16; 290.42: 25766-81.
PMID: 26316537
A Rhizobium radiobacter Histidine Kinase Can Employ Both Boolean AND and OR Logic Gates to Initiate Pathogenesis.
Chembiochem : a European journal of chemical biology. 2015-10-12; 16.15: 2183-90.
PMID: 26310519
Role of the VirA histidine autokinase of Agrobacterium tumefaciens in the initial steps of pathogenesis.
Frontiers in plant science. 2014-05-14; 5.195.
PMID: 24860585
The receiver domain of hybrid histidine kinase VirA: an enhancing factor for vir gene expression in Agrobacterium tumefaciens.
Journal of bacteriology. 2010-03-01; 192.6: 1534-42.
PMID: 20081031
Structural characterization of sialic acid synthase by electrospray mass spectrometry--a tetrameric enzyme composed of dimeric dimers.
Journal of the American Society for Mass Spectrometry. 2005-03-01; 16.3: 324-32.
PMID: 15734325