Niu B, Fu L, Sun S, Li W

Artificial and natural duplicates in pyrosequencing reads of metagenomic data.

BMC bioinformatics. 2010-04-13; 11.187.

Artificial duplicates from pyrosequencing reads may lead to incorrect interpretation of the abundance of species and genes in metagenomic studies. Duplicated reads were filtered out in many metagenomic projects. However, since the duplicated reads observed in a pyrosequencing run also include natural (non-artificial) duplicates, simply removing all duplicates may also cause underestimation of abundance associated with natural duplicates.

PMID: 20388221

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