Ding L, Laor D, Weisman R, Forsburg SL
Rapid regulation of nuclear proteins by rapamycin-induced translocation in fission yeast.
Yeast (Chichester, England). 2014-07-01; 31.7: 253-64.
Genetic analysis of protein function requires a rapid means of inactivating the gene under study. Typically, this exploits temperature-sensitive mutations or promoter shut-off techniques. We report the adaptation to Schizosaccharomyces pombe of the anchor-away technique, originally designed in budding yeast by Laemmli lab. This method relies on a rapamycin-mediated interaction between the FRB- and FKBP12-binding domains to relocalize nuclear proteins of interest to the cytoplasm. We demonstrate a rapid nuclear depletion of abundant proteins as proof of principle.
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