Chemical synthesis of the mouse mitochondrial genome.

Chemical synthesis of the mouse mitochondrial genome.

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Authors: Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C
Title: Chemical synthesis of the mouse mitochondrial genome.
Citation: Nature methods. 2010-11-01; 7.11: 901-3.
Abstract:
We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.
PMID: 20935651