Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C
Chemical synthesis of the mouse mitochondrial genome.
Nature methods. 2010-11-01; 7.11: 901-3.
We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.