Publications

Bioinformatics (Oxford, England). 2001-12-01; 17.12: 1093-104.

DNA sequence quality trimming and vector removal

Chou HH, Holmes MH

PMID: 11751217

Abstract

Most sequence comparison methods assume that the data being compared are trustworthy, but this is not the case with raw DNA sequences obtained from automatic sequencing machines. Nevertheless, sequence comparisons need to be done on them in order to remove vector splice sites and contaminants. This step is necessary before other genomic data processing stages can be carried out, such as fragment assembly or EST clustering. A specialized tool is therefore needed to solve this apparent dilemma.

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