Publications

Methods in molecular biology (Clifton, N.J.). 2014-01-01; 1140.61-74.

High-throughput cloning for biophysical applications

Kwon K, Peterson SN

PMID: 24590709

Abstract

Efficient high-throughput gene cloning represents a critical first step for conducting functional and structural proteomics in the post-genomic era. The ligation-independent cloning (LIC) method has been almost universally adopted by large structural biology centers as a component of high-throughput structure determination pipelines. The LIC platform is easy to use, of low cost, and rapid, and importantly, it is easily adapted to 96- or 384-well format, thereby facilitating automation. Procedures are described for 96-well format cloning using the LIC technology.

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