Improved method for selecting RNA-binding activities in vivo.

Improved method for selecting RNA-binding activities in vivo.

You are here

Authors: Fouts DE, Celander DW
Title: Improved method for selecting RNA-binding activities in vivo.
Citation: Nucleic acids research. 1996-04-15; 24.8: 1582-4.
Abstract:
RNA challenge phages are modified versions of bacteriophage P22 that allow one to select directly for a specific RNA-protein interaction in vivo. The original construction method for generating a bacteriophage that encodes a specific RNA target requires two homologous recombination reactions between plasmids and phages in bacteria. An improved method is described that enables one to readily construct RNA challenge phages through a single homologous recombination reaction in vivo. We have applied the new method to construct a derivative of P22R17, an RNA challenge phage that undergoes lysogenic development in bacterial cells that express the bacteriophage R17/MS2 coat protein.
PMID: 8628698

This publication is listed for reference purposes only. It may be included to present a more complete view of a JCVI employee's body of work, or as a reference to a JCVI sponsored project.