Publications

iScience. 2026-09-18; 29.9: 117211.

Mycoplasma mycoides subsp. capri encodes multiple pathways for hydrogen peroxide production

Chandran S, Gonzalez-Juarbe N, Assad-Garcia N, Yimthin T, Démoulins T, Zhang J, Alperovich N, Chuang RY, Falquet L, Blanchard A, Sirand-Pugnet P, Lartigue C, Jores J, Vashee S

PMID: 42667075

Abstract

Several species import glycerol and related molecules as carbon sources, resulting in the release of hydrogen peroxide (HO), which has been suggested as a pathogenicity mechanism. We systematically deleted genes encoding components of the HO pathways in subsp. GM12 to get insight into the role of candidate factors constituting the pathways. As expected, glycerol metabolism in GM12 is associated with the highest HO production compared with glycerophosphocholine. The absence of either L-alpha-glycerophosphate oxidase (GlpO) or the glycerol uptake facilitator GlpF, which is the main glycerol importer in GM12, ceased HO production. However, our data did not support any role for the presumed glycerol transport system, GtsABC in glycerol- or glycerophosphocholine-dependent HO production. Caprine peripheral blood mononuclear cells (PBMCs) did not show increased cytotoxic effects upon incubation with GM12 in the presence of glycerol, which does not support HO to be a primary pathogenicity mechanism in GM12.

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