Plasma membrane repair is mediated by Ca(2+)-regulated exocytosis of lysosomes.

Plasma membrane repair is mediated by Ca(2+)-regulated exocytosis of lysosomes.

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Authors: Reddy A, Caler EV, Andrews NW
Title: Plasma membrane repair is mediated by Ca(2+)-regulated exocytosis of lysosomes.
Citation: Cell. 2001-07-27; 106.2: 157-69.
Abstract:
Plasma membrane wounds are repaired by a mechanism involving Ca(2+)-regulated exocytosis. Elevation in intracellular [Ca(2+)] triggers fusion of lysosomes with the plasma membrane, a process regulated by the lysosomal synaptotagmin isoform Syt VII. Here, we show that Ca(2+)-regulated exocytosis of lysosomes is required for the repair of plasma membrane disruptions. Lysosomal exocytosis and membrane resealing are inhibited by the recombinant Syt VII C(2)A domain or anti-Syt VII C(2)A antibodies, or by antibodies against the cytosolic domain of Lamp-1, which specifically aggregate lysosomes. We further demonstrate that lysosomal exocytosis mediates the resealing of primary skin fibroblasts wounded during the contraction of collagen matrices. These findings reveal a fundamental, novel role for lysosomes: as Ca(2+)-regulated exocytic compartments responsible for plasma membrane repair.
PMID: 11511344