Publications

STAR protocols. 2026-08-05; 7.3: 104726.

Protocol to investigate the role of NETosis in health and disease via a high-throughput neutrophil-like model using quantitative imaging

Li G, Versales S, Guadagnino B, Krishnan K, Matsuoka M, Jang H, Freire M

PMID: 42560812

Abstract

Neutrophil extracellular traps (NETs) are a crucial host-defense mechanism employed by neutrophils in response to various aggregates and contribute to pathology in diverse diseases, from chronic inflammation to sepsis. Here, we present a protocol to investigate the role of NETosis, the release of NETs, in health and disease via a high-throughput neutrophil-like model using quantitative imaging. We describe steps for preparing stimulated, differentiated Human Leukemia-60 (HL-60) cells; immunostaining; high-throughput microscopy; and NETosis quantification using CellProfiler.

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