Publications

BMC genomics. 2005-03-01; 6.27.

A robust method for the amplification of RNA in the sense orientation

Marko NF, Frank B, Quackenbush J, Lee NH

PMID: 15740627

Abstract

Small quantities of RNA (1-4 microg total RNA) available from biological samples frequently require a single round of amplification prior to analysis, but current amplification strategies have limitations that may restrict their usefulness in downstream genomic applications. The Eberwine amplification method has been extensively validated but is limited by its ability to produce only antisense RNA. Alternatives lack extensive validation and are often confounded by problems with bias or yield attributable to their greater biological and technical complexity.

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