Sequencing viral genomes from a single isolated plaque.

Sequencing viral genomes from a single isolated plaque.

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Authors: Depew J, Zhou B, McCorrison JM, Wentworth DE, Purushe J, Koroleva G, Fouts DE
Title: Sequencing viral genomes from a single isolated plaque.
Citation: Virology journal. 2013-06-06; 10.: 181.
Abstract:
Whole genome sequencing of viruses and bacteriophages is often hindered because of the need for large quantities of genomic material. A method is described that combines single plaque sequencing with an optimization of Sequence Independent Single Primer Amplification (SISPA). This method can be used for de novo whole genome next-generation sequencing of any cultivable virus without the need for large-scale production of viral stocks or viral purification using centrifugal techniques.
PMID: 23742765