Burkholderia Genome Sequencing and Analysis

We developed some of the earliest annotated genome sequences for the genus Burkholderia including the biothreat agents Burkholderia mallei and Burkholderia pseudomallei. We expanded on our genome sequencing efforts to include efforts in whole genome gene expression analysis and functional analysis of genome variation. We also sequenced several members of the Burkholderia cenocepacia complex that are important pathogens in the lungs of cystic fibrosis patients.


Frontiers in microbiology. 2015-01-01; 6.1338.
The In vitro Antibiotic Tolerant Persister Population in Burkholderia pseudomallei is Altered by Environmental Factors
Nierman WC, Yu Y, Losada L
PMID: 26696964
PLoS genetics. 2014-09-01; 10.9: e1004640.
The tandem repeats enabling reversible switching between the two phases of β-lactamase substrate spectrum
Yi H, Song H, Hwang J, Kim K, Nierman WC, Kim HS
PMID: 25233343
Genome announcements. 2013-11-07; 1.6:
Sequence Determination of Burkholderia pseudomallei Strain NCTC 13392 Colony Morphology Variants
Vipond J, Kane J, Hatch G, McCorrison J, Nierman WC, Losada L
PMID: 24201202
Genome announcements. 2013-10-17; 1.5:
Draft Genome Sequences of Burkholderia cenocepacia ET12 Lineage Strains K56-2 and BC7
Varga JJ, Losada L, Zelazny AM, Kim M, McCorrison J, Brinkac L, Sampaio EP, Greenberg DE, Singh I, Heiner C, Ashby M, Nierman WC, Holland SM, Goldberg JB
PMID: 24136849
Journal of bacteriology. 2012-11-01; 194.22: 6356-7.
Draft genome sequence determination for cystic fibrosis and chronic granulomatous disease Burkholderia multivorans isolates
Varga JJ, Losada L, Zelazny AM, Brinkac L, Harkins D, Radune D, Hostetler J, Sampaio EP, Ronning CM, Nierman WC, Greenberg DE, Holland SM, Goldberg JB
PMID: 23105085
PloS one. 2012-05-22; 7.5: e37585.
Twelve positions in a β-lactamase that can expand its substrate spectrum with a single amino acid substitution
Yi H, Cho KH, Cho YS, Kim K, Nierman WC, Kim HS
PMID: 22629423
Molecules and cells. 2009-02-28; 27.2: 237-41.
Simple sequence repeat (SSR)-based gene diversity in Burkholderia pseudomallei and Burkholderia mallei
Song H, Hwang J, Myung J, Seo H, Yi H, Sim HS, Kim BS, Nierman WC, Kim HS
PMID: 19277507
BMC genomics. 2008-11-27; 9.566.
Genomic islands from five strains of Burkholderia pseudomallei
Tuanyok A, Leadem BR, Auerbach RK, Beckstrom-Sternberg SM, Beckstrom-Sternberg JS, Mayo M, Wuthiekanun V, Brettin TS, Nierman WC, Peacock SJ, Currie BJ, Wagner DM, Keim P
PMID: 19038032
PloS one. 2008-04-30; 3.4: e2058.
Characterization of clinically-attenuated Burkholderia mallei by whole genome sequencing: candidate strain for exclusion from Select Agent lists
Schutzer SE, Schlater LR, Ronning CM, DeShazer D, Luft BJ, Dunn JJ, Ravel J, Fraser-Liggett CM, Nierman WC
PMID: 18446194


Funding for this project provided through the National Institutes of Health, National Institute of Allergy and Infectious Diseases (NIAID).

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