Urinary Proteomics for Host-Pathogen Interaction Analysis and Diagnosis of Urinary Tract Infections

Urinary metaproteomics of urine sediments can be used to characterize host-pathogen interactions and diagnose urinary tract infections and non-infectious inflammatory conditions of the urinary system. Urinary tract infections may be caused by one or multiple microbial species. The interplay of opportunistic pathogens of the urinary and vaginal tracts, commensal microbes at the urinary and vaginal tract meatus and the human host will be elucidated.

Despite the prevalence of asymptomatic bacteriuria and urinary tract infections, the course of bacterial colonization of the urinary tract and the molecular-physiological consequences of human host immune system reactions towards the microbial challenge and progression towards disease symptoms are not fully understood.

State-of-the-art metagenomics and metaproteomics analysis techniques now allow profiling the complexity of such interactions among the bacteria that colonize the urinary tract and those that cause disease symptoms, as well as the respective inflammatory and microbicidal responses of the human host in more depth. We collaborate with Shady Grove Adventist Hospital, Rockville, MD. The objectives are to understand host-pathogen interactions and to determine if the methods are of diagnostic value to provide an alternative to commonly used urine analysis and urine culture tests.


Scientific reports. 2018-03-12; 8.1: 4333.
Microbial metagenome of urinary tract infection
Moustafa A, Li W, Singh H, Moncera KJ, Torralba MG, Yu Y, Manuel O, Biggs W, Venter JC, Nelson KE, Pieper R, Telenti A
PMID: 29531289
PLoS pathogens. 2017-01-27; 13.1: e1006151.
Characterization of Early-Phase Neutrophil Extracellular Traps in Urinary Tract Infections
Yu Y, Kwon K, Tsitrin T, Bekele S, Sikorski P, Nelson KE, Pieper R
PMID: 28129394
Theranostics. 2017-01-01; 7.2: 238-252.
Comprehensive Metaproteomic Analyses of Urine in the Presence and Absence of Neutrophil-Associated Inflammation in the Urinary Tract
Yu Y, Sikorski P, Smith M, Bowman-Gholston C, Cacciabeve N, Nelson KE, Pieper R
PMID: 28042331
Infection and immunity. 2015-10-01; 83.10: 4142-53.
Similar Neutrophil-Driven Inflammatory and Antibacterial Responses in Elderly Patients with Symptomatic and Asymptomatic Bacteriuria
Yu Y, Zielinski MD, Rolfe MA, Kuntz MM, Nelson H, Nelson KE, Pieper R
PMID: 26238715
Journal of translational medicine. 2015-04-08; 13.111.
Diagnosing inflammation and infection in the urinary system via proteomics
Yu Y, Sikorski P, Bowman-Gholston C, Cacciabeve N, Nelson KE, Pieper R
PMID: 25889401
Analytical chemistry. 2014-06-03; 86.11: 5470-7.
Urine sample preparation in 96-well filter plates for quantitative clinical proteomics
Yu Y, Suh MJ, Sikorski P, Kwon K, Nelson KE, Pieper R
PMID: 24797144


This project is funded, in part, by the National Institute of Health, award NIH-1R01GM103598-01.


Shady Grove Adventist Hospital

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