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High-resolution BAC-based map of the central portion of mouse chromosome 5.
The current strategy for sequencing the mouse genome involves the combination of a whole-genome shotgun approach with clone-based sequencing. High-resolution physical maps will provide a foundation for assembling contiguous segments of sequence. We have established a bacterial artificial chromosome (BAC)-based map of a 5-Mb region on mouse Chromosome 5, encompassing three gene families: receptor tyrosine kinases (PdgfraKit-Kdr), nonreceptor protein-tyrosine type kinases (Tec-Txk), and...
Scientists Decipher the Rat Genome
March 31, 2004 Rockville, MD — An international consortium of scientists, including The Institute for Genomic Research (TIGR), has completed a high-quality draft sequence of the rat genome. The project provides a valuable resource for researchers who use laboratory rats in their investigations of experimental medicines to treat human diseases. The study — the primary results of which appear in Nature's April 1 issue — found that nearly all human genes known to be...
Sequence-tagged connectors: a sequence approach to mapping and scanning the human genome.
The sequence-tagged connector (STC) strategy proposes to generate sequence tags densely scattered (every 3.3 kilobases) across the human genome by arraying 450,000 bacterial artificial chromosomes (BACs) with randomly cleaved inserts, sequencing both ends of each, and preparing a restriction enzyme fingerprint of each. The STC resource, containing end sequences, fingerprints, and arrayed BACs, creates a map where the interrelationships of the individual BAC clones are resolved through their...
TIGR to Participate in Sequencing the Rat Genome
February 28, 2001 ROCKVILLE, MD -The Institute for Genomic Research (TIGR), a not-for-profit research organization, announced it is part of a new collaboration to sequence the genome of the laboratory rat. The project is established and funded by the National Heart, Lung, and Blood Institute (NHLBI) and the National Human Genome Research Institute (NHGRI). Divisions of the National Institutes of Health (NIH), NHLBI and NHGRI awarded two new grants to the Baylor College of Medicine...
Construction, characterization, and preliminary BAC-end sequencing analysis of a bacterial artificial chromosome library of white clover (Trifolium repens L.).
White clover (Trifolium repens L.) is a forage legume widely used in combination with grass in pastures because of its ability to fix nitrogen. We have constructed a bacterial artificial chromosome (BAC) library of an advanced breeding line of white clover. The library contains 37 248 clones with an average insert size of approximately 85 kb, representing an approximate 3-fold coverage of the white clover genome based on an estimated genome size of 960 Mb. The BAC library was pooled and...
Segments missing from the draft human genome sequence can be isolated by transformation-associated recombination cloning in yeast.
The reported draft human genome sequence includes many contigs that are separated by gaps of unknown sequence. These gaps may be due to chromosomal regions that are not present in the Escherichia coli libraries used for DNA sequencing because they cannot be cloned efficiently, if at all, in bacteria. Using a yeast artificial chromosome (YAC)/ bacterial artificial chromosome (BAC) library generated in yeast, we found that approximately 6% of human DNA sequences tested transformed E. coli...
Scientists Decipher Genetic Code of Malaria Parasite
October 2, 2002 Rockville, MD - In a landmark contribution to the age-old battle against malaria, a consortium of scientists including The Institute for Genomic Research (TIGR) announced today that they have deciphered the complex genetic code of the parasite that causes the deadliest form of the disease. Malaria is one of the world's most devastating infectious diseases, killing more than a million people a year in developing nations. The scientific paper analyzing the genome of that...