Publications

BMC biotechnology. 2018-06-01; 18.1: 37.

DNA assembly with error correction on a droplet digital microfluidics platform

Khilko Y, Weyman PD, Glass JI, Adams MD, McNeil MA, Griffin PB

PMID: 29859085

Abstract

Custom synthesized DNA is in high demand for synthetic biology applications. However, current technologies to produce these sequences using assembly from DNA oligonucleotides are costly and labor-intensive. The automation and reduced sample volumes afforded by microfluidic technologies could significantly decrease materials and labor costs associated with DNA synthesis. The purpose of this study was to develop a gene assembly protocol utilizing a digital microfluidic device. Toward this goal, we adapted bench-scale oligonucleotide assembly methods followed by enzymatic error correction to the Mondrian™ digital microfluidic platform.

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