Sensitive and specific method for detecting G protein-coupled receptor mRNAs.

Sensitive and specific method for detecting G protein-coupled receptor mRNAs.

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Authors: Hansen A, Chen Y, Inman JM, Phan QN, Qi ZQ, Xiang CC, Palkovits M, Cherman N, Kuznetsov SA, Robey PG, Mezey E, Brownstein MJ
Title: Sensitive and specific method for detecting G protein-coupled receptor mRNAs.
Citation: Nature methods. 2007-01-01; 4.1: 35-7.
Abstract:
G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.
PMID: 17115035