BREAD: Toward Development of a Vaccine for Contagious Bovine Pleuropneumonia (CBPP)

Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subspecies mycoides (Mmm), is an economically very important livestock disease within Africa that limits the availability of protein sources for nutrition and restricts trade. The current control relies on a live vaccine that has shortcomings, such as, limited efficacy and short duration of immunity. International efforts to create a vaccine to protect against CBPP have been thwarted because of an almost complete lack of genetic tools for this species. As a result, it has been difficult to apply the tools of modern biology to this pressing African need. In this project, the J. Craig Venter Institute (JCVI) joins forces with the International Livestock Research Institute (ILRI) in Kenya and the National Institute for Agronomical Research (INRA) in France to explore the use of new synthetic biology technologies to genetically manipulate Mmm genomes with the ultimate goal of creating strains that can be developed as live vaccine derivatives and identifying virulence traits using subsequent in vitro and in vivo assays.

These JCVI synthetic biology approaches are a transformational advance in our ability to design and engineer novel bacteria with extraordinary properties to serve human needs. Since previous JCVI work including the creation of the synthetic cell was done using a closely related species to Mmm, Mycoplasma mycoides subsp. capri (Mmc), and synthetic biology tools were developed for this species, JCVI will continue its work with Mmc during the course of this project. INRA will develop the synthetic biology tools for Mmm as well as the pneumonia-causing strain of Mmc and ILRI will implement the tools and test mutants using in vitro and in vivo assays.

While our long-term perspective is to use key synthetic biology technologies to produce a safe and efficient Mmm vaccine strain by rationally modifying its genome, the current project is meant to lay the foundation for the development of a modern CBPP vaccine. Together, we will expand the mycoplasma toolbox using Mmc as a model in order to enhance our capacity to produce modern Mmm vaccines. We will also establish a caprine model for pulmonary mycoplasma infections with the available tools using the closely related pathogen Mmc.


Nucleic acids research. 2016-09-30; 44.17: 8501-11.
Impact of donor-recipient phylogenetic distance on bacterial genome transplantation
Labroussaa F, Lebaudy A, Baby V, Gourgues G, Matteau D, Vashee S, Sirand-Pugnet P, Rodrigue S, Lartigue C
PMID: 27488189
Proceedings of the National Academy of Sciences of the United States of America. 2016-05-10; 113.19: 5406-11.
MIB-MIP is a mycoplasma system that captures and cleaves immunoglobulin G
Arfi Y, Minder L, Di Primo C, Le Roy A, Ebel C, Coquet L, Claverol S, Vashee S, Jores J, Blanchard A, Sirand-Pugnet P
PMID: 27114507
Genome announcements. 2016-04-14; 4.2:
Complete Genome Sequence of Mycoplasma mycoides subsp. mycoides T1/44, a Vaccine Strain against Contagious Bovine Pleuropneumonia
Gourgues G, Barré A, Beaudoing E, Weber J, Magdelenat G, Barbe V, Schieck E, Jores J, Vashee S, Blanchard A, Lartigue C, Sirand-Pugnet P
PMID: 27081135
ACS synthetic biology. 2016-01-15; 5.1: 104-9.
In-Yeast Engineering of a Bacterial Genome Using CRISPR/Cas9
Tsarmpopoulos I, Gourgues G, Blanchard A, Vashee S, Jores J, Lartigue C, Sirand-Pugnet P
PMID: 26592087
Molecular microbiology. 2016-01-01; 99.1: 55-70.
Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance
Schieck E, Lartigue C, Frey J, Vozza N, Hegermann J, Miller RA, Valguarnera E, Muriuki C, Meens J, Nene V, Naessens J, Weber J, Lowary TL, Vashee S, Feldman MF, Jores J
PMID: 26354009
Standards in genomic sciences. 2015-10-29; 10.89.
High quality draft genomes of the Mycoplasma mycoides subsp. mycoides challenge strains Afadé and B237
Fischer A, Santana-Cruz I, Hegerman J, Gourlé H, Schieck E, Lambert M, Nadendla S, Wesonga H, Miller RA, Vashee S, Weber J, Meens J, Frey J, Jores J
PMID: 26516405
BMC genomics. 2014-12-24; 15.1180.
TREC-IN: gene knock-in genetic tool for genomes cloned in yeast
Chandran S, Noskov VN, Segall-Shapiro TH, Ma L, Whiteis C, Lartigue C, Jores J, Vashee S, Chuang RY
PMID: 25539750
Current opinion in biotechnology. 2012-10-01; 23.5: 659-65.
Synthetic genomics: potential and limitations
Montague MG, Lartigue C, Vashee S
PMID: 22342755


Funding for this project provided by NSF-BREAD IOS 1110151.

Principal Investigator

Key Staff


Carole Lartigue
INRA, France

Joerg Jores
University of Bern, Switzerland


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