Clyde A. Hutchison, III, PhD

Distinguished Professor

Clyde Hutchison, III, PhD, is a Distinguished Professor at the J. Craig Venter Institute in La Jolla, California, where he is a member of the Synthetic Biology Group. He is also a consultant for Synthetic Genomics, Inc. In 1995 he was elected to membership in the National Academy of Sciences. He graduated from Yale University in 1960, with a BS in Physics. His graduate studies were in the laboratory of Robert L. Sinsheimer at Cal Tech where he finished his PhD in 1968. He was a member of the faculty of The University of North Carolina at Chapel Hill from 1968 until 2005, where he now holds the title Kenan Professor Emeritus. He has worked on the molecular genetics of bacteriophage, bacteria, and mammals. In Fred Sanger's lab (1975-6) he helped determine the first complete sequence of a DNA molecule (phiX174). He developed site-directed mutagenesis with Michael Smith (1978). In 1990 he began work with mycoplasmas as models for the minimal cell. This led to collaboration with Smith and Venter, and his current work on synthetic genomics.

Research Priorities

Understanding the minimal requirements for life
  • Determining functions of all genes in the minimal cell, JCVI-syn3.0.
Developing methods for genome design
  • Extending the design of minimized genomes to simple eukaryotes.
Modeling the minimal cell
  • Collaborating with other labs to build a predictive computer model of JCVI-syn3.0.

Publications

Gross Chromosomal Rearrangements in Kluyveromyces marxianus Revealed by Illumina and Oxford Nanopore Sequencing.
International journal of molecular sciences. 2020-09-26; 21.19:
PMID: 32993167
Kinetic Modeling of the Genetic Information Processes in a Minimal Cell.
Frontiers in molecular biosciences. 2019-11-28; 6.130.
PMID: 31850364
Polar Effects of Transposon Insertion into a Minimal Bacterial Genome.
Journal of bacteriology. 2019-10-01; 201.19:
PMID: 31262838
Tuning Gene Activity by Inducible and Targeted Regulation of Gene Expression in Minimal Bacterial Cells.
ACS synthetic biology. 2018-06-15; 7.6: 1538-1552.
PMID: 29786424
Minimal Cells-Real and Imagined.
Cold Spring Harbor perspectives in biology. 2017-12-01; 9.12:
PMID: 28348033
One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9.
Scientific reports. 2016-08-04; 6.30714.
PMID: 27489041
Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling.
Genome research. 2015-03-01; 25.3: 435-44.
PMID: 25654978
Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing.
Nature protocols. 2014-04-01; 9.4: 743-50.
PMID: 24603933
Rescue of mutant fitness defects using in vitro reconstituted designer transposons in Mycoplasma mycoides.
Frontiers in microbiology. 2014-01-01; 5.369.
PMID: 25101070
Assembly of eukaryotic algal chromosomes in yeast.
Journal of biological engineering. 2013-12-10; 7.1: 30.
PMID: 24325901
Direct transfer of whole genomes from bacteria to yeast.
Nature methods. 2013-05-01; 10.5: 410-2.
PMID: 23542886
Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast.
Nucleic acids research. 2012-11-01; 40.20: 10375-83.
PMID: 22941652
Assembly of large, high G+C bacterial DNA fragments in yeast.
ACS synthetic biology. 2012-07-20; 1.7: 267-73.
PMID: 23651249
Chemical synthesis of the mouse mitochondrial genome.
Nature methods. 2010-11-01; 7.11: 901-3.
PMID: 20935651
Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Science (New York, N.Y.). 2009-09-25; 325.5948: 1693-6.
PMID: 19696314
Enzymatic assembly of DNA molecules up to several hundred kilobases.
Nature methods. 2009-05-01; 6.5: 343-5.
PMID: 19363495
A systems biology tour de force for a near-minimal bacterium.
Molecular systems biology. 2009-01-01; 5.330.
PMID: 19953084
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome.
Proceedings of the National Academy of Sciences of the United States of America. 2008-12-23; 105.51: 20404-9.
PMID: 19073939
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Science (New York, N.Y.). 2008-02-29; 319.5867: 1215-20.
PMID: 18218864
Genome transplantation in bacteria: changing one species to another.
Science (New York, N.Y.). 2007-08-03; 317.5838: 632-8.
PMID: 17600181
DNA sequencing: bench to bedside and beyond.
Nucleic acids research. 2007-01-01; 35.Database issue: 6227-37.
PMID: 17855400
Cell-free cloning using phi29 DNA polymerase.
Proceedings of the National Academy of Sciences of the United States of America. 2005-11-29; 102.48: 17332-6.
PMID: 16286637
Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides.
Proceedings of the National Academy of Sciences of the United States of America. 2003-12-23; 100.26: 15440-5.
PMID: 14657399
Global transposon mutagenesis and a minimal Mycoplasma genome.
Science (New York, N.Y.). 1999-12-10; 286.5447: 2165-9.
PMID: 10591650
A survey of the Mycoplasma genitalium genome by using random sequencing.
Journal of bacteriology. 1993-12-01; 175.24: 7918-30.
PMID: 8253680
The sequence of a large L1Md element reveals a tandemly repeated 5' end and several features found in retrotransposons.
Molecular and cellular biology. 1986-01-01; 6.1: 168-82.
PMID: 3023821
Mutagenesis at a specific position in a DNA sequence.
The Journal of biological chemistry. 1978-09-25; 253.18: 6551-60.
PMID: 681366
Overlapping genes in bacteriophage phiX174.
Nature. 1976-11-04; 264.5581: 34-41.
PMID: 1004533
Maternal inheritance of mammalian mitochondrial DNA.
Nature. 1974-10-11; 251.5475: 536-8.
PMID: 4423884

Research Priorities

Understanding the minimal requirements for life
  • Determining functions of all genes in the minimal cell, JCVI-syn3.0.
Developing methods for genome design
  • Extending the design of minimized genomes to simple eukaryotes.
Modeling the minimal cell
  • Collaborating with other labs to build a predictive computer model of JCVI-syn3.0.
20-Aug-2009
Press Release

J. Craig Venter Institute Researchers Clone and Engineer Bacterial Genomes in Yeast and Transplant Genomes Back into Bacterial Cells

New methods allow for the rapid engineering of bacterial chromosomes and the creation of extensively modified bacterial species; should also play key role in boot up of synthetic cell

24-Jan-2008
Press Release

Venter Institute Scientists Create First Synthetic Bacterial Genome

Publication Represents Largest Chemically Defined Structure Synthesized in the Lab

Team Completes Second Step in Three Step Process to Create Synthetic Organism

28-Jun-2007
Press Release

JCVI Scientists Publish First Bacterial Genome Transplantation Changing One Species to Another

Research is important step in further advancing field of synthetic genomics

13-Nov-2003
Press Release

IBEA Researchers Make Significant Advance in Methodology Toward Goal of a Synthetic Genome

Group Synthesizes Biologically Active Genome of Bacteriophage φX174

IBEA research, funded by Dept. of Energy, is an important advance toward the goal of a completely synthetic genome that could aid in carbon sequestration and energy production