Hamilton Smith, MD

Distinguished Professor Emeritus

Hamilton Smith, M.D. is a distinguished professor emeritus at the J. Craig Venter Institute and former director of the Synthetic Biology and Bioenergy Group that created the first synthetic cell in 2010 and designed and synthesized the first minimal bacterial genome in 2016. He received an A.B. degree in Mathematics from the University of California, Berkeley in 1952 and the M.D. degree from the Johns Hopkins School of Medicine in 1956. He completed a residency in Internal Medicine in 1962. From 1962-67, he did research on lysogeny in phage P22 at the University of Michigan. In 1967, he was appointed Assistant Professor of Microbiology at Johns Hopkins. In 1970, he discovered the first Type II restriction enzyme, HindII. He received the Nobel Prize in Medicine in 1978 for that work. From 1975 to 1998, he studied the DNA transformation mechanism in Haemophilus Inflluenzae Rd. In 1993, Dr. Smith began his long association with Craig Venter. In 1995, they collaborated to sequence the genome of H. Inflluenzae Rd, and from 1998 to 2002, at Celera Genomics they sequenced Drosophila and the human genome. After leaving Celera, Smith and Venter began their work in synthetic biology.

Research Priorities

Deciphering the genetic requirements for minimal bacterial life
  • Assignment of genetic functions to unknown genes in the minimal cell by computational, biochemical and genetic methods.
Designing a minimal yeast cell
  • Determining non-essential genes by global DNA cassette insertional mutagenesis.
Understanding the mechanism of DNA cassette insertional mutagenesis in yeast
  • Genetic and biochemical analysis of the DNA insertion mechanism.

Publications

Cell. 2022-01-20; 185.2: 345-360.e28.
Fundamental behaviors emerge from simulations of a living minimal cell
Thornburg ZR, Bianchi DM, Brier TA, Gilbert BR, Earnest TM, Melo MCR, Safronova N, Sáenz JP, Cook AT, Wise KS, Hutchison CA, Smith HO, Glass JI, Luthey-Schulten Z
PMID: 35063075
International journal of molecular sciences. 2020-09-26; 21.19:
Gross Chromosomal Rearrangements in Kluyveromyces marxianus Revealed by Illumina and Oxford Nanopore Sequencing
Ding L, Macdonald HD, Smith HO, Hutchison Iii CA, Merryman C, Michael TP, Abramson BW, Kannan K, Liang J, Gill J, Gibson DG, Glass JI
PMID: 32993167
Frontiers in molecular biosciences. 2019-11-28; 6.130.
Kinetic Modeling of the Genetic Information Processes in a Minimal Cell
Thornburg ZR, Melo MCR, Bianchi D, Brier TA, Crotty C, Breuer M, Smith HO, Hutchison CA, Glass JI, Luthey-Schulten Z
PMID: 31850364
Journal of bacteriology. 2019-10-01; 201.19:
Polar Effects of Transposon Insertion into a Minimal Bacterial Genome
Hutchison CA, Merryman C, Sun L, Assad-Garcia N, Richter RA, Smith HO, Glass JI
PMID: 31262838
eLife. 2019-01-18; 8.
Essential metabolism for a minimal cell
Breuer M, Earnest TM, Merryman C, Wise KS, Sun L, Lynott MR, Hutchison CA, Smith HO, Lapek JD, Gonzalez DJ, de Crécy-Lagard V, Haas D, Hanson AD, Labhsetwar P, Glass JI, Luthey-Schulten Z
PMID: 30657448
ACS synthetic biology. 2019-01-15;
Direct transfer of a Mycoplasma mycoides genome to yeast is enhanced by removal of the mycoides glycerol uptake factor gene glpF
Karas BJ, Moreau N, Deernick TJ, Gibson DG, Venter JC, Smith H, Glass J
PMID: 30645947
ACS synthetic biology. 2018-06-15; 7.6: 1538-1552.
Tuning Gene Activity by Inducible and Targeted Regulation of Gene Expression in Minimal Bacterial Cells
Mariscal AM, Kakizawa S, Hsu JY, Tanaka K, González-González L, Broto A, Querol E, Lluch-Senar M, Piñero-Lambea C, Sun L, Weyman PD, Wise KS, Merryman C, Tse G, Moore AJ, Hutchison CA, Smith HO, Tomita M, Venter JC, Glass JI, Piñol J, Suzuki Y
PMID: 29786424
Cold Spring Harbor perspectives in biology. 2017-12-01; 9.12:
Minimal Cells-Real and Imagined
Glass JI, Merryman C, Wise KS, Hutchison CA, Smith HO
PMID: 28348033
Scientific reports. 2016-08-04; 6.30714.
One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9
Kannan K, Tsvetanova B, Chuang RY, Noskov VN, Assad-Garcia N, Ma L, Hutchison Iii CA, Smith HO, Glass JI, Merryman C, Venter JC, Gibson DG
PMID: 27489041
Science (New York, N.Y.). 2016-03-25; 351.6280: aad6253.
Design and synthesis of a minimal bacterial genome
Hutchison CA, Chuang RY, Noskov VN, Assad-Garcia N, Deerinck TJ, Ellisman MH, Gill J, Kannan K, Karas BJ, Ma L, Pelletier JF, Qi ZQ, Richter RA, Strychalski EA, Sun L, Suzuki Y, Tsvetanova B, Wise KS, Smith HO, Glass JI, Merryman C, Gibson DG, Venter JC
PMID: 27013737
Clinical chemistry. 2015-09-01; 61.9: 1207-8.
The Sequence of the Human Genome
Venter JC, Smith HO, Adams MD
PMID: 26185218
Nature communications. 2015-04-21; 6.6925.
Designer diatom episomes delivered by bacterial conjugation
Karas BJ, Diner RE, Lefebvre SC, McQuaid J, Phillips AP, Noddings CM, Brunson JK, Valas RE, Deerinck TJ, Jablanovic J, Gillard JT, Beeri K, Ellisman MH, Glass JI, Hutchison CA, Smith HO, Venter JC, Allen AE, Dupont CL, Weyman PD
PMID: 25897682
Genome research. 2015-03-01; 25.3: 435-44.
Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling
Suzuki Y, Assad-Garcia N, Kostylev M, Noskov VN, Wise KS, Karas BJ, Stam J, Montague MG, Hanly TJ, Enriquez NJ, Ramon A, Goldgof GM, Richter RA, Vashee S, Chuang RY, Winzeler EA, Hutchison CA, Gibson DG, Smith HO, Glass JI, Venter JC
PMID: 25654978
PLoS biology. 2015-01-20; 13.1: e1002050.
Where next for microbiome research?
Waldor MK, Tyson G, Borenstein E, Ochman H, Moeller A, Finlay BB, Kong HH, Gordon JI, Nelson KE, Dabbagh K, Smith H
PMID: 25602283
International journal of molecular sciences. 2015-01-16; 16.1: 2020-33.
Designed surface residue substitutions in [NiFe] hydrogenase that improve electron transfer characteristics
Yonemoto IT, Smith HO, Weyman PD
PMID: 25603181
BMC biochemistry. 2014-06-17; 15.10.
A broad survey reveals substitution tolerance of residues ligating FeS clusters in [NiFe] hydrogenase
Yonemoto IT, Clarkson BR, Smith HO, Weyman PD
PMID: 24934472
Nature protocols. 2014-04-01; 9.4: 743-50.
Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing
Karas BJ, Jablanovic J, Irvine E, Sun L, Ma L, Weyman PD, Gibson DG, Glass JI, Venter JC, Hutchison CA, Smith HO, Suzuki Y
PMID: 24603933
Frontiers in microbiology. 2014-01-01; 5.369.
Rescue of mutant fitness defects using in vitro reconstituted designer transposons in Mycoplasma mycoides
Karas BJ, Wise KS, Sun L, Venter JC, Glass JI, Hutchison CA, Smith HO, Suzuki Y
PMID: 25101070
Journal of biological engineering. 2013-12-10; 7.1: 30.
Assembly of eukaryotic algal chromosomes in yeast
Karas BJ, Molparia B, Jablanovic J, Hermann WJ, Lin YC, Dupont CL, Tagwerker C, Yonemoto IT, Noskov VN, Chuang RY, Allen AE, Glass JI, Hutchison CA, Smith HO, Venter JC, Weyman PD
PMID: 24325901
Journal of biological engineering. 2013-07-02; 7.1: 17.
Dual organism design cycle reveals small subunit substitutions that improve [NiFe] hydrogenase hydrogen evolution
Yonemoto IT, Matteri CW, Nguyen TA, Smith HO, Weyman PD
PMID: 23819621
Nature methods. 2013-05-01; 10.5: 410-2.
Direct transfer of whole genomes from bacteria to yeast
Karas BJ, Jablanovic J, Sun L, Ma L, Goldgof GM, Stam J, Ramon A, Manary MJ, Winzeler EA, Venter JC, Weyman PD, Gibson DG, Glass JI, Hutchison CA, Smith HO, Suzuki Y
PMID: 23542886
Nucleic acids research. 2012-11-01; 40.20: 10375-83.
Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast
Tagwerker C, Dupont CL, Karas BJ, Ma L, Chuang RY, Benders GA, Ramon A, Novotny M, Montague MG, Venepally P, Brami D, Schwartz A, Andrews-Pfannkoch C, Gibson DG, Glass JI, Smith HO, Venter JC, Hutchison CA
PMID: 22941652
ACS synthetic biology. 2012-07-20; 1.7: 267-73.
Assembly of large, high G+C bacterial DNA fragments in yeast
Noskov VN, Karas BJ, Young L, Chuang RY, Gibson DG, Lin YC, Stam J, Yonemoto IT, Suzuki Y, Andrews-Pfannkoch C, Glass JI, Smith HO, Hutchison CA, Venter JC, Weyman PD
PMID: 23651249
FEMS microbiology letters. 2011-09-01; 322.2: 180-7.
Genetic analysis of the Alteromonas macleodii [NiFe]-hydrogenase
Weyman PD, Smith HO, Xu Q
PMID: 21718346
Microbiology (Reading, England). 2011-05-01; 157.Pt 5: 1363-74.
Heterologous expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] hydrogenases in Escherichia coli
Weyman PD, Vargas WA, Chuang RY, Chang Y, Smith HO, Xu Q
PMID: 21349975
Applied and environmental microbiology. 2011-03-01; 77.6: 1990-8.
[NiFe] hydrogenase from Alteromonas macleodii with unusual stability in the presence of oxygen and high temperature
Vargas WA, Weyman PD, Tong Y, Smith HO, Xu Q
PMID: 21257809
Biotechnology letters. 2011-03-01; 33.3: 549-55.
Single-step linker-based combinatorial assembly of promoter and gene cassettes for pathway engineering
Ramon A, Smith HO
PMID: 21107654
Nature methods. 2010-11-01; 7.11: 901-3.
Chemical synthesis of the mouse mitochondrial genome
Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C
PMID: 20935651
Science (New York, N.Y.). 2010-07-02; 329.5987: 52-6.
Creation of a bacterial cell controlled by a chemically synthesized genome
Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, Merryman C, Vashee S, Krishnakumar R, Assad-Garcia N, Andrews-Pfannkoch C, Denisova EA, Young L, Qi ZQ, Segall-Shapiro TH, Calvey CH, Parmar PP, Hutchison CA, Smith HO, Venter JC
PMID: 20488990
Nucleic acids research. 2010-05-01; 38.8: 2558-69.
Cloning whole bacterial genomes in yeast
Benders GA, Noskov VN, Denisova EA, Lartigue C, Gibson DG, Assad-Garcia N, Chuang RY, Carrera W, Moodie M, Algire MA, Phan Q, Alperovich N, Vashee S, Merryman C, Venter JC, Smith HO, Glass JI, Hutchison CA
PMID: 20211840
Journal of molecular evolution. 2009-10-01; 69.4: 360-71.
The evolution of RecD outside of the RecBCD complex
Montague M, Barnes C, Smith HO, Chuang RY, Vashee S
PMID: 19841849
Science (New York, N.Y.). 2009-09-25; 325.5948: 1693-6.
Creating bacterial strains from genomes that have been cloned and engineered in yeast
Lartigue C, Vashee S, Algire MA, Chuang RY, Benders GA, Ma L, Noskov VN, Denisova EA, Gibson DG, Assad-Garcia N, Alperovich N, Thomas DW, Merryman C, Hutchison CA, Smith HO, Venter JC, Glass JI
PMID: 19696314
Applied and environmental microbiology. 2009-09-01; 75.18: 5821-30.
Discovery of [NiFe] hydrogenase genes in metagenomic DNA: cloning and heterologous expression in Thiocapsa roseopersicina
Maróti G, Tong Y, Yooseph S, Baden-Tillson H, Smith HO, Kovács KL, Frazier M, Venter JC, Xu Q
PMID: 19633107
Nature methods. 2009-05-01; 6.5: 343-5.
Enzymatic assembly of DNA molecules up to several hundred kilobases
Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO
PMID: 19363495
Molecular systems biology. 2009-01-01; 5.330.
A systems biology tour de force for a near-minimal bacterium
Glass JI, Hutchison CA, Smith HO, Venter JC
PMID: 19953084
Proceedings of the National Academy of Sciences of the United States of America. 2008-12-23; 105.51: 20404-9.
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
Gibson DG, Benders GA, Axelrod KC, Zaveri J, Algire MA, Moodie M, Montague MG, Venter JC, Smith HO, Hutchison CA
PMID: 19073939
Science (New York, N.Y.). 2008-02-29; 319.5867: 1215-20.
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome
Gibson DG, Benders GA, Andrews-Pfannkoch C, Denisova EA, Baden-Tillson H, Zaveri J, Stockwell TB, Brownley A, Thomas DW, Algire MA, Merryman C, Young L, Noskov VN, Glass JI, Venter JC, Hutchison CA, Smith HO
PMID: 18218864
Science (New York, N.Y.). 2007-08-03; 317.5838: 632-8.
Genome transplantation in bacteria: changing one species to another
Lartigue C, Glass JI, Alperovich N, Pieper R, Parmar PP, Hutchison CA, Smith HO, Venter JC
PMID: 17600181
PLoS biology. 2007-03-01; 5.3: e77.
The Sorcerer II Global Ocean Sampling expedition: northwest Atlantic through eastern tropical Pacific
Rusch DB, Halpern AL, Sutton G, Heidelberg KB, Williamson S, Yooseph S, Wu D, Eisen JA, Hoffman JM, Remington K, Beeson K, Tran B, Smith H, Baden-Tillson H, Stewart C, Thorpe J, Freeman J, Andrews-Pfannkoch C, Venter JE, Li K, Kravitz S, Heidelberg JF, Utterback T, Rogers YH, Falcón LI, Souza V, Bonilla-Rosso G, Eguiarte LE, Karl DM, Sathyendranath S, Platt T, Bermingham E, Gallardo V, Tamayo-Castillo G, Ferrari MR, Strausberg RL, Nealson K, Friedman R, Frazier M, Venter JC
PMID: 17355176
Proceedings of the National Academy of Sciences of the United States of America. 2006-01-10; 103.2: 425-30.
Essential genes of a minimal bacterium
Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA, Smith HO, Venter JC
PMID: 16407165
Proceedings of the National Academy of Sciences of the United States of America. 2005-11-29; 102.48: 17332-6.
Cell-free cloning using phi29 DNA polymerase
Hutchison CA, Smith HO, Pfannkoch C, Venter JC
PMID: 16286637
Science (New York, N.Y.). 2004-04-02; 304.5667: 66-74.
Environmental genome shotgun sequencing of the Sargasso Sea
Venter JC, Remington K, Heidelberg JF, Halpern AL, Rusch D, Eisen JA, Wu D, Paulsen I, Nelson KE, Nelson W, Fouts DE, Levy S, Knap AH, Lomas MW, Nealson K, White O, Peterson J, Hoffman J, Parsons R, Baden-Tillson H, Pfannkoch C, Rogers YH, Smith HO
PMID: 15001713
Proceedings of the National Academy of Sciences of the United States of America. 2003-12-23; 100.26: 15440-5.
Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides
Smith HO, Hutchison CA, Pfannkoch C, Venter JC
PMID: 14657399
Nature biotechnology. 2002-11-01; 20.11: 1118-23.
Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis
Heidelberg JF, Paulsen IT, Nelson KE, Gaidos EJ, Nelson WC, Read TD, Eisen JA, Seshadri R, Ward N, Methe B, Clayton RA, Meyer T, Tsapin A, Scott J, Beanan M, Brinkac L, Daugherty S, Deboy RT, Dodson RJ, Durkin AS, Haft DH, Kolonay JF, Madupu R, Peterson JD, Umayam LA, White O, Wolf AM, Vamathevan J, Weidman J, Impraim M, Lee K, Berry K, Lee C, Mueller J, Khouri H, Gill J, Utterback TR, McDonald LA, Feldblyum TV, Smith HO, Venter JC, Nealson KH, Fraser CM
PMID: 12368813
Science (New York, N.Y.). 2002-10-04; 298.5591: 129-49.
The genome sequence of the malaria mosquito Anopheles gambiae
Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R, Salzberg SL, Loftus B, Yandell M, Majoros WH, Rusch DB, Lai Z, Kraft CL, Abril JF, Anthouard V, Arensburger P, Atkinson PW, Baden H, de Berardinis V, Baldwin D, Benes V, Biedler J, Blass C, Bolanos R, Boscus D, Barnstead M, Cai S, Center A, Chaturverdi K, Christophides GK, Chrystal MA, Clamp M, Cravchik A, Curwen V, Dana A, Delcher A, Dew I, Evans CA, Flanigan M, Grundschober-Freimoser A, Friedli L, Gu Z, Guan P, Guigo R, Hillenmeyer ME, Hladun SL, Hogan JR, Hong YS, Hoover J, Jaillon O, Ke Z, Kodira C, Kokoza E, Koutsos A, Letunic I, Levitsky A, Liang Y, Lin JJ, Lobo NF, Lopez JR, Malek JA, McIntosh TC, Meister S, Miller J, Mobarry C, Mongin E, Murphy SD, O'Brochta DA, Pfannkoch C, Qi R, Regier MA, Remington K, Shao H, Sharakhova MV, Sitter CD, Shetty J, Smith TJ, Strong R, Sun J, Thomasova D, Ton LQ, Topalis P, Tu Z, Unger MF, Walenz B, Wang A, Wang J, Wang M, Wang X, Woodford KJ, Wortman JR, Wu M, Yao A, Zdobnov EM, Zhang H, Zhao Q, Zhao S, Zhu SC, Zhimulev I, Coluzzi M, della Torre A, Roth CW, Louis C, Kalush F, Mural RJ, Myers EW, Adams MD, Smith HO, Broder S, Gardner MJ, Fraser CM, Birney E, Bork P, Brey PT, Venter JC, Weissenbach J, Kafatos FC, Collins FH, Hoffman SL
PMID: 12364791
Nature. 2002-10-03; 419.6906: 531-4.
Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14
Gardner MJ, Shallom SJ, Carlton JM, Salzberg SL, Nene V, Shoaibi A, Ciecko A, Lynn J, Rizzo M, Weaver B, Jarrahi B, Brenner M, Parvizi B, Tallon L, Moazzez A, Granger D, Fujii C, Hansen C, Pederson J, Feldblyum T, Peterson J, Suh B, Angiuoli S, Pertea M, Allen J, Selengut J, White O, Cummings LM, Smith HO, Adams MD, Venter JC, Carucci DJ, Hoffman SL, Fraser CM
PMID: 12368868
Nature. 2002-10-03; 419.6906: 512-9.
Genome sequence and comparative analysis of the model rodent malaria parasite Plasmodium yoelii yoelii
Carlton JM, Angiuoli SV, Suh BB, Kooij TW, Pertea M, Silva JC, Ermolaeva MD, Allen JE, Selengut JD, Koo HL, Peterson JD, Pop M, Kosack DS, Shumway MF, Bidwell SL, Shallom SJ, Van Aken SE, Riedmuller SB, Feldblyum TV, Cho JK, Quackenbush J, Sedegah M, Shoaibi A, Cummings LM, Florens L, Yates JR, Raine JD, Sinden RE, Harris MA, Cunningham DA, Preiser PR, Bergman LW, Vaidya AB, van Lin LH, Janse CJ, Waters AP, Smith HO, White OR, Salzberg SL, Venter JC, Fraser CM, Hoffman SL, Gardner MJ, Carucci DJ
PMID: 12368865
Science (New York, N.Y.). 2002-05-31; 296.5573: 1661-71.
A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome
Mural RJ, Adams MD, Myers EW, Smith HO, Miklos GL, Wides R, Halpern A, Li PW, Sutton GG, Nadeau J, Salzberg SL, Holt RA, Kodira CD, Lu F, Chen L, Deng Z, Evangelista CC, Gan W, Heiman TJ, Li J, Li Z, Merkulov GV, Milshina NV, Naik AK, Qi R, Shue BC, Wang A, Wang J, Wang X, Yan X, Ye J, Yooseph S, Zhao Q, Zheng L, Zhu SC, Biddick K, Bolanos R, Delcher AL, Dew IM, Fasulo D, Flanigan MJ, Huson DH, Kravitz SA, Miller JR, Mobarry CM, Reinert K, Remington KA, Zhang Q, Zheng XH, Nusskern DR, Lai Z, Lei Y, Zhong W, Yao A, Guan P, Ji RR, Gu Z, Wang ZY, Zhong F, Xiao C, Chiang CC, Yandell M, Wortman JR, Amanatides PG, Hladun SL, Pratts EC, Johnson JE, Dodson KL, Woodford KJ, Evans CA, Gropman B, Rusch DB, Venter E, Wang M, Smith TJ, Houck JT, Tompkins DE, Haynes C, Jacob D, Chin SH, Allen DR, Dahlke CE, Sanders R, Li K, Liu X, Levitsky AA, Majoros WH, Chen Q, Xia AC, Lopez JR, Donnelly MT, Newman MH, Glodek A, Kraft CL, Nodell M, Ali F, An HJ, Baldwin-Pitts D, Beeson KY, Cai S, Carnes M, Carver A, Caulk PM, Center A, Chen YH, Cheng ML, Coyne MD, Crowder M, Danaher S, Davenport LB, Desilets R, Dietz SM, Doup L, Dullaghan P, Ferriera S, Fosler CR, Gire HC, Gluecksmann A, Gocayne JD, Gray J, Hart B, Haynes J, Hoover J, Howland T, Ibegwam C, Jalali M, Johns D, Kline L, Ma DS, MacCawley S, Magoon A, Mann F, May D, McIntosh TC, Mehta S, Moy L, Moy MC, Murphy BJ, Murphy SD, Nelson KA, Nuri Z, Parker KA, Prudhomme AC, Puri VN, Qureshi H, Raley JC, Reardon MS, Regier MA, Rogers YH, Romblad DL, Schutz J, Scott JL, Scott R, Sitter CD, Smallwood M, Sprague AC, Stewart E, Strong RV, Suh E, Sylvester K, Thomas R, Tint NN, Tsonis C, Wang G, Wang G, Williams MS, Williams SM, Windsor SM, Wolfe K, Wu MM, Zaveri J, Chaturvedi K, Gabrielian AE, Ke Z, Sun J, Subramanian G, Venter JC, Pfannkoch CM, Barnstead M, Stephenson LD
PMID: 12040188
Proceedings of the National Academy of Sciences of the United States of America. 2002-04-02; 99.7: 4145-6.
On the sequencing and assembly of the human genome
Myers EW, Sutton GG, Smith HO, Adams MD, Venter JC
PMID: 11904395
Science (New York, N.Y.). 2001-07-20; 293.5529: 498-506.
Complete genome sequence of a virulent isolate of Streptococcus pneumoniae
Tettelin H, Nelson KE, Paulsen IT, Eisen JA, Read TD, Peterson S, Heidelberg J, Deboy RT, Haft DH, Dodson RJ, Durkin AS, Gwinn M, Kolonay JF, Nelson WC, Peterson JD, Umayam LA, White O, Salzberg SL, Lewis MR, Radune D, Holtzapple E, Khouri H, Wolf AM, Utterback TR, Hansen CL, McDonald LA, Feldblyum TV, Angiuoli S, Dickinson T, Hickey EK, Holt IE, Loftus BJ, Yang F, Smith HO, Venter JC, Dougherty BA, Morrison DA, Hollingshead SK, Fraser CM
PMID: 11463916
Science (New York, N.Y.). 2001-02-16; 291.5507: 1304-51.
The sequence of the human genome
Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, Smith HO, Yandell M, Evans CA, Holt RA, Gocayne JD, Amanatides P, Ballew RM, Huson DH, Wortman JR, Zhang Q, Kodira CD, Zheng XH, Chen L, Skupski M, Subramanian G, Thomas PD, Zhang J, Gabor Miklos GL, Nelson C, Broder S, Clark AG, Nadeau J, McKusick VA, Zinder N, Levine AJ, Roberts RJ, Simon M, Slayman C, Hunkapiller M, Bolanos R, Delcher A, Dew I, Fasulo D, Flanigan M, Florea L, Halpern A, Hannenhalli S, Kravitz S, Levy S, Mobarry C, Reinert K, Remington K, Abu-Threideh J, Beasley E, Biddick K, Bonazzi V, Brandon R, Cargill M, Chandramouliswaran I, Charlab R, Chaturvedi K, Deng Z, Di Francesco V, Dunn P, Eilbeck K, Evangelista C, Gabrielian AE, Gan W, Ge W, Gong F, Gu Z, Guan P, Heiman TJ, Higgins ME, Ji RR, Ke Z, Ketchum KA, Lai Z, Lei Y, Li Z, Li J, Liang Y, Lin X, Lu F, Merkulov GV, Milshina N, Moore HM, Naik AK, Narayan VA, Neelam B, Nusskern D, Rusch DB, Salzberg S, Shao W, Shue B, Sun J, Wang Z, Wang A, Wang X, Wang J, Wei M, Wides R, Xiao C, Yan C, Yao A, Ye J, Zhan M, Zhang W, Zhang H, Zhao Q, Zheng L, Zhong F, Zhong W, Zhu S, Zhao S, Gilbert D, Baumhueter S, Spier G, Carter C, Cravchik A, Woodage T, Ali F, An H, Awe A, Baldwin D, Baden H, Barnstead M, Barrow I, Beeson K, Busam D, Carver A, Center A, Cheng ML, Curry L, Danaher S, Davenport L, Desilets R, Dietz S, Dodson K, Doup L, Ferriera S, Garg N, Gluecksmann A, Hart B, Haynes J, Haynes C, Heiner C, Hladun S, Hostin D, Houck J, Howland T, Ibegwam C, Johnson J, Kalush F, Kline L, Koduru S, Love A, Mann F, May D, McCawley S, McIntosh T, McMullen I, Moy M, Moy L, Murphy B, Nelson K, Pfannkoch C, Pratts E, Puri V, Qureshi H, Reardon M, Rodriguez R, Rogers YH, Romblad D, Ruhfel B, Scott R, Sitter C, Smallwood M, Stewart E, Strong R, Suh E, Thomas R, Tint NN, Tse S, Vech C, Wang G, Wetter J, Williams S, Williams M, Windsor S, Winn-Deen E, Wolfe K, Zaveri J, Zaveri K, Abril JF, Guigó R, Campbell MJ, Sjolander KV, Karlak B, Kejariwal A, Mi H, Lazareva B, Hatton T, Narechania A, Diemer K, Muruganujan A, Guo N, Sato S, Bafna V, Istrail S, Lippert R, Schwartz R, Walenz B, Yooseph S, Allen D, Basu A, Baxendale J, Blick L, Caminha M, Carnes-Stine J, Caulk P, Chiang YH, Coyne M, Dahlke C, Mays A, Dombroski M, Donnelly M, Ely D, Esparham S, Fosler C, Gire H, Glanowski S, Glasser K, Glodek A, Gorokhov M, Graham K, Gropman B, Harris M, Heil J, Henderson S, Hoover J, Jennings D, Jordan C, Jordan J, Kasha J, Kagan L, Kraft C, Levitsky A, Lewis M, Liu X, Lopez J, Ma D, Majoros W, McDaniel J, Murphy S, Newman M, Nguyen T, Nguyen N, Nodell M, Pan S, Peck J, Peterson M, Rowe W, Sanders R, Scott J, Simpson M, Smith T, Sprague A, Stockwell T, Turner R, Venter E, Wang M, Wen M, Wu D, Wu M, Xia A, Zandieh A, Zhu X
PMID: 11181995
Journal of molecular biology. 2000-08-04; 301.1: 27-33.
Prediction of transcription terminators in bacterial genomes
Ermolaeva MD, Khalak HG, White O, Smith HO, Salzberg SL
PMID: 10926490
Nature. 2000-08-03; 406.6795: 477-83.
DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae
Heidelberg JF, Eisen JA, Nelson WC, Clayton RA, Gwinn ML, Dodson RJ, Haft DH, Hickey EK, Peterson JD, Umayam L, Gill SR, Nelson KE, Read TD, Tettelin H, Richardson D, Ermolaeva MD, Vamathevan J, Bass S, Qin H, Dragoi I, Sellers P, McDonald L, Utterback T, Fleishmann RD, Nierman WC, White O, Salzberg SL, Smith HO, Colwell RR, Mekalanos JJ, Venter JC, Fraser CM
PMID: 10952301
Science (New York, N.Y.). 2000-03-24; 287.5461: 2185-95.
The genome sequence of Drosophila melanogaster
Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, Scherer SE, Li PW, Hoskins RA, Galle RF, George RA, Lewis SE, Richards S, Ashburner M, Henderson SN, Sutton GG, Wortman JR, Yandell MD, Zhang Q, Chen LX, Brandon RC, Rogers YH, Blazej RG, Champe M, Pfeiffer BD, Wan KH, Doyle C, Baxter EG, Helt G, Nelson CR, Gabor GL, Abril JF, Agbayani A, An HJ, Andrews-Pfannkoch C, Baldwin D, Ballew RM, Basu A, Baxendale J, Bayraktaroglu L, Beasley EM, Beeson KY, Benos PV, Berman BP, Bhandari D, Bolshakov S, Borkova D, Botchan MR, Bouck J, Brokstein P, Brottier P, Burtis KC, Busam DA, Butler H, Cadieu E, Center A, Chandra I, Cherry JM, Cawley S, Dahlke C, Davenport LB, Davies P, de Pablos B, Delcher A, Deng Z, Mays AD, Dew I, Dietz SM, Dodson K, Doup LE, Downes M, Dugan-Rocha S, Dunkov BC, Dunn P, Durbin KJ, Evangelista CC, Ferraz C, Ferriera S, Fleischmann W, Fosler C, Gabrielian AE, Garg NS, Gelbart WM, Glasser K, Glodek A, Gong F, Gorrell JH, Gu Z, Guan P, Harris M, Harris NL, Harvey D, Heiman TJ, Hernandez JR, Houck J, Hostin D, Houston KA, Howland TJ, Wei MH, Ibegwam C, Jalali M, Kalush F, Karpen GH, Ke Z, Kennison JA, Ketchum KA, Kimmel BE, Kodira CD, Kraft C, Kravitz S, Kulp D, Lai Z, Lasko P, Lei Y, Levitsky AA, Li J, Li Z, Liang Y, Lin X, Liu X, Mattei B, McIntosh TC, McLeod MP, McPherson D, Merkulov G, Milshina NV, Mobarry C, Morris J, Moshrefi A, Mount SM, Moy M, Murphy B, Murphy L, Muzny DM, Nelson DL, Nelson DR, Nelson KA, Nixon K, Nusskern DR, Pacleb JM, Palazzolo M, Pittman GS, Pan S, Pollard J, Puri V, Reese MG, Reinert K, Remington K, Saunders RD, Scheeler F, Shen H, Shue BC, Sidén-Kiamos I, Simpson M, Skupski MP, Smith T, Spier E, Spradling AC, Stapleton M, Strong R, Sun E, Svirskas R, Tector C, Turner R, Venter E, Wang AH, Wang X, Wang ZY, Wassarman DA, Weinstock GM, Weissenbach J, Williams SM, WoodageT, Worley KC, Wu D, Yang S, Yao QA, Ye J, Yeh RF, Zaveri JS, Zhan M, Zhang G, Zhao Q, Zheng L, Zheng XH, Zhong FN, Zhong W, Zhou X, Zhu S, Zhu X, Smith HO, Gibbs RA, Myers EW, Rubin GM, Venter JC
PMID: 10731132
Science (New York, N.Y.). 2000-03-10; 287.5459: 1809-15.
Complete genome sequence of Neisseria meningitidis serogroup B strain MC58
Tettelin H, Saunders NJ, Heidelberg J, Jeffries AC, Nelson KE, Eisen JA, Ketchum KA, Hood DW, Peden JF, Dodson RJ, Nelson WC, Gwinn ML, DeBoy R, Peterson JD, Hickey EK, Haft DH, Salzberg SL, White O, Fleischmann RD, Dougherty BA, Mason T, Ciecko A, Parksey DS, Blair E, Cittone H, Clark EB, Cotton MD, Utterback TR, Khouri H, Qin H, Vamathevan J, Gill J, Scarlato V, Masignani V, Pizza M, Grandi G, Sun L, Smith HO, Fraser CM, Moxon ER, Rappuoli R, Venter JC
PMID: 10710307
Science (New York, N.Y.). 1999-12-10; 286.5447: 2165-9.
Global transposon mutagenesis and a minimal Mycoplasma genome
Hutchison CA, Peterson SN, Gill SR, Cline RT, White O, Fraser CM, Smith HO, Venter JC
PMID: 10591650
Science (New York, N.Y.). 1999-11-19; 286.5444: 1571-7.
Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1
White O, Eisen JA, Heidelberg JF, Hickey EK, Peterson JD, Dodson RJ, Haft DH, Gwinn ML, Nelson WC, Richardson DL, Moffat KS, Qin H, Jiang L, Pamphile W, Crosby M, Shen M, Vamathevan JJ, Lam P, McDonald L, Utterback T, Zalewski C, Makarova KS, Aravind L, Daly MJ, Minton KW, Fleischmann RD, Ketchum KA, Nelson KE, Salzberg S, Smith HO, Venter JC, Fraser CM
PMID: 10567266
Research in microbiology. 1999-11-01; 150.9-10: 603-16.
DNA uptake signal sequences in naturally transformable bacteria
Smith HO, Gwinn ML, Salzberg SL
PMID: 10673000
Parassitologia. 1999-09-01; 41.1-3: 69-75.
The malaria genome sequencing project: complete sequence of Plasmodium falciparum chromosome 2
Gardner MJ, Tettelin H, Carucci DJ, Cummings LM, Smith HO, Fraser CM, Venter JC, Hoffman SL
PMID: 10697835
Nature. 1999-05-27; 399.6734: 323-9.
Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima
Nelson KE, Clayton RA, Gill SR, Gwinn ML, Dodson RJ, Haft DH, Hickey EK, Peterson JD, Nelson WC, Ketchum KA, McDonald L, Utterback TR, Malek JA, Linher KD, Garrett MM, Stewart AM, Cotton MD, Pratt MS, Phillips CA, Richardson D, Heidelberg J, Sutton GG, Fleischmann RD, Eisen JA, White O, Salzberg SL, Smith HO, Venter JC, Fraser CM
PMID: 10360571
Science (New York, N.Y.). 1998-11-06; 282.5391: 1126-32.
Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum
Gardner MJ, Tettelin H, Carucci DJ, Cummings LM, Aravind L, Koonin EV, Shallom S, Mason T, Yu K, Fujii C, Pederson J, Shen K, Jing J, Aston C, Lai Z, Schwartz DC, Pertea M, Salzberg S, Zhou L, Sutton GG, Clayton R, White O, Smith HO, Fraser CM, Adams MD, Venter JC, Hoffman SL
PMID: 9804551
Science (New York, N.Y.). 1998-07-17; 281.5375: 375-88.
Complete genome sequence of Treponema pallidum, the syphilis spirochete
Fraser CM, Norris SJ, Weinstock GM, White O, Sutton GG, Dodson R, Gwinn M, Hickey EK, Clayton R, Ketchum KA, Sodergren E, Hardham JM, McLeod MP, Salzberg S, Peterson J, Khalak H, Richardson D, Howell JK, Chidambaram M, Utterback T, McDonald L, Artiach P, Bowman C, Cotton MD, Fujii C, Garland S, Hatch B, Horst K, Roberts K, Sandusky M, Weidman J, Smith HO, Venter JC
PMID: 9665876
Science (New York, N.Y.). 1998-06-05; 280.5369: 1540-2.
Shotgun sequencing of the human genome
Venter JC, Adams MD, Sutton GG, Kerlavage AR, Smith HO, Hunkapiller M
PMID: 9644018
Journal of bacteriology. 1998-02-01; 180.3: 746-8.
A new transformation-deficient mutant of Haemophilus influenzae Rd with normal DNA uptake
Gwinn ML, Ramanathan R, Smith HO, Tomb JF
PMID: 9457884
Nature. 1997-12-11; 390.6660: 580-6.
Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi
Fraser CM, Casjens S, Huang WM, Sutton GG, Clayton R, Lathigra R, White O, Ketchum KA, Dodson R, Hickey EK, Gwinn M, Dougherty B, Tomb JF, Fleischmann RD, Richardson D, Peterson J, Kerlavage AR, Quackenbush J, Salzberg S, Hanson M, van Vugt R, Palmer N, Adams MD, Gocayne J, Weidman J, Utterback T, Watthey L, McDonald L, Artiach P, Bowman C, Garland S, Fuji C, Cotton MD, Horst K, Roberts K, Hatch B, Smith HO, Venter JC
PMID: 9403685
Journal of bacteriology. 1997-12-01; 179.23: 7315-20.
In vitro Tn7 mutagenesis of Haemophilus influenzae Rd and characterization of the role of atpA in transformation
Gwinn ML, Stellwagen AE, Craig NL, Tomb JF, Smith HO
PMID: 9393695
Nature. 1997-11-27; 390.6658: 364-70.
The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus
Klenk HP, Clayton RA, Tomb JF, White O, Nelson KE, Ketchum KA, Dodson RJ, Gwinn M, Hickey EK, Peterson JD, Richardson DL, Kerlavage AR, Graham DE, Kyrpides NC, Fleischmann RD, Quackenbush J, Lee NH, Sutton GG, Gill S, Kirkness EF, Dougherty BA, McKenney K, Adams MD, Loftus B, Peterson S, Reich CI, McNeil LK, Badger JH, Glodek A, Zhou L, Overbeek R, Gocayne JD, Weidman JF, McDonald L, Utterback T, Cotton MD, Spriggs T, Artiach P, Kaine BP, Sykes SM, Sadow PW, D'Andrea KP, Bowman C, Fujii C, Garland SA, Mason TM, Olsen GJ, Fraser CM, Smith HO, Woese CR, Venter JC
PMID: 9389475
Nature. 1997-08-07; 388.6642: 539-47.
The complete genome sequence of the gastric pathogen Helicobacter pylori
Tomb JF, White O, Kerlavage AR, Clayton RA, Sutton GG, Fleischmann RD, Ketchum KA, Klenk HP, Gill S, Dougherty BA, Nelson K, Quackenbush J, Zhou L, Kirkness EF, Peterson S, Loftus B, Richardson D, Dodson R, Khalak HG, Glodek A, McKenney K, Fitzegerald LM, Lee N, Adams MD, Hickey EK, Berg DE, Gocayne JD, Utterback TR, Peterson JD, Kelley JM, Cotton MD, Weidman JM, Fujii C, Bowman C, Watthey L, Wallin E, Hayes WS, Borodovsky M, Karp PD, Smith HO, Fraser CM, Venter JC
PMID: 9252185
Nature. 1996-05-30; 381.6581: 364-6.
A new strategy for genome sequencing
Venter JC, Smith HO, Hood L
PMID: 8632789
Science (New York, N.Y.). 1995-10-20; 270.5235: 397-403.
The minimal gene complement of Mycoplasma genitalium
Fraser CM, Gocayne JD, White O, Adams MD, Clayton RA, Fleischmann RD, Bult CJ, Kerlavage AR, Sutton G, Kelley JM, Fritchman RD, Weidman JF, Small KV, Sandusky M, Fuhrmann J, Nguyen D, Utterback TR, Saudek DM, Phillips CA, Merrick JM, Tomb JF, Dougherty BA, Bott KF, Hu PC, Lucier TS, Peterson SN, Smith HO, Hutchison CA, Venter JC
PMID: 7569993
Science (New York, N.Y.). 1995-07-28; 269.5223: 538-40.
Frequency and distribution of DNA uptake signal sequences in the Haemophilus influenzae Rd genome
Smith HO, Tomb JF, Dougherty BA, Fleischmann RD, Venter JC
PMID: 7542802
Science (New York, N.Y.). 1995-07-28; 269.5223: 496-512.
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd
Fleischmann RD, Adams MD, White O, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM
PMID: 7542800
Biotechnology (Reading, Mass.). 1992-01-01; 24.38-50.
A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. 1970
Smith HO, Wilcox KW
PMID: 1330118
Proceedings of the National Academy of Sciences of the United States of America. 1990-01-01; 87.2: 826-30.
Finding sequence motifs in groups of functionally related proteins
Smith HO, Annau TM, Chandrasegaran S
PMID: 1689055
Journal of molecular biology. 1970-07-28; 51.2: 393-409.
A restriction enzyme from Hemophilus influenzae. II
Kelly TJ, Smith HO
PMID: 5312501
Salk Intitute Lecture — 2016 DOE JGI Genomics of Energy & Environment Meeting
Johns Hopkins Medicine — The Hamilton Smith Award for Innovative Research
UW-Madison Lecture
Salk Intitute Lecture — Making miniature machines - with Hamilton Smith
The Nobel Prize — Interview with Hamilton Smith and Richard Roberts
22-Dec-2020
Press Release

Hamilton O. Smith, M.D., Synthetic Biology Pioneer and Nobel Laureate, to Step Down from Daily Duties at J. Craig Venter Institute

Dr. Smith will maintain advisory role as professor emeritus

19-Dec-2020
The San Diego Union-Tribune

After saving countless lives, Nobel laureate Hamilton Smith retires as his own health falters

He has been a fixture in San Diego science for decades

13-Jun-2012
Press Release

JCVI Researchers, as Part of NIH Human Microbiome Project Consortium, Publish Papers Detailing the Variety and Abundance of Microbes Living on and in the Human Body

Study Represents Largest Group of Healthy Individuals Studied to Date

JCVI also Details its Metagenomics Reports (METAREP) Open Source Bioinformatics Tool

20-Aug-2009
Press Release

J. Craig Venter Institute Researchers Clone and Engineer Bacterial Genomes in Yeast and Transplant Genomes Back into Bacterial Cells

New methods allow for the rapid engineering of bacterial chromosomes and the creation of extensively modified bacterial species; should also play key role in boot up of synthetic cell

24-Jan-2008
Press Release

Venter Institute Scientists Create First Synthetic Bacterial Genome

Publication Represents Largest Chemically Defined Structure Synthesized in the Lab

Team Completes Second Step in Three Step Process to Create Synthetic Organism

28-Jun-2007
Press Release

JCVI Scientists Publish First Bacterial Genome Transplantation Changing One Species to Another

Research is important step in further advancing field of synthetic genomics

29-Sep-2004
Press Release

J. Craig Venter Announces Consolidation of Three Research Organizations Into One New Not-For-Profit Organization — The J. Craig Venter Institute

The Center for Advancement of Genomics (TCAG), Institute for Biological Energy Alternatives (IBEA), and J. Craig Venter Science Foundation Joint Technology Center (JTC) consolidated to form J. Craig Venter Institute

13-Nov-2003
Press Release

IBEA Researchers Make Significant Advance in Methodology Toward Goal of a Synthetic Genome

Group Synthesizes Biologically Active Genome of Bacteriophage φX174

IBEA research, funded by Dept. of Energy, is an important advance toward the goal of a completely synthetic genome that could aid in carbon sequestration and energy production

21-Nov-2002
Press Release

IBEA Receives $3 Million Dept. of Energy Grant for Synthetic Genome Development

Hamilton Smith, M.D., Nobel Laureate, Named Scientific Director of IBEA